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AIM: To investigate the influencing factors of disequilibrium refractive development in children aged 6-12 years old.METHODS: A total of 607 children with a mean age of 8.2±1.8 years old from two primary schools in Binhai New Area of Tianjin in December 2019 were selected by cluster sampling for optical biometry measurement(axial length and equivalent corneal power), cycloplegia refractive examination and questionnaire survey.RESULTS: The childrens'spherical equivalent was -0.11±1.63D, binocular spherical equivalent difference was -0.08±0.64D and absolute value of spherical equivalent difference was 0.41±0.49D. There were 56 children with anisometropia and 551 children without anisometropia. There was no difference in the absolute value of binocular corneal power difference between anisometropia and non-anisometropia group(0.30±0.34D vs 0.27±0.24D, P=0.430). But the absolute value of axial difference was significantly different(0.67±0.39mm vs 0.13±0.13mm, P=0.005). Multivariate linear regression analysis showed that weekly usage time of phones/computers, weekly close work hours, weekly visual fatigue times and habitual reading distance were the influencing factors of refractive imbalance development(the absolute value of binocular spherical equivalent difference). Habitual reading distance and handedness in writing are the influencing factors of the developmental bias of refractive imbalance(binocular spherical equivalent difference).CONCLUSION: Children aged 6-12 years old who spend more time on phones/computers, and have prolonged close work, more times of visual fatigue and close habitual reading distance may increase the degree of refractive imbalance. Children with right-handed handwriting and severe left or right head deviation have a deeper degree of myopia in the right eye than in the left eye.
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A quantitative analytical method based on HPLC coupled with the charged aerosol detector (CAD) for quantitative analysis of multi-components with a single marker (QAMS) was established for simultaneous determinations of astragaloside Ⅰ, astragaloside Ⅱ, astragaloside Ⅳ, calycosin-7-O-β-D-glucoside, formononetin and 7,2'-dihydroxy-3',4'-dimethoxyisoflavan in Astragalus membranaceus. The separation was performed on an Agilent SB-C18 (150 mm×4.6 mm, 3.5 μm), with gradient elution using the mobile phase consisting of 0.05% formic acid solution and 0.05% formic acid acetonitrile at the flow rate of 1.0 mL·min-1. The column temperature was 35 ℃, and the injection volume was 20 μL. For CAD, the drift tube temperature was at 50 ℃. The contents of six components in A. membranaceus were determined by both external standard method (ESM) and QAMS, and then were compared. The results showed that chromatographic peaks were separated well and the linear ranges of astragaloside Ⅰ, astragaloside Ⅱ, astragaloside Ⅳ, calycosin-7-glucoside, formononetin and 7,2'-dihydroxy-3',4'-dimethoxyisoflavan were 0.113-2.250 mg·mL-1, 0.012-0.240 mg·mL-1, 0.004-0.080 mg·mL-1, 0.065-1.300 mg·mL-1, 0.005-0.100 mg·mL-1 and 0.007-0.150 mg·mL-1, respectively. The content ranges of astragaloside Ⅰ, astragaloside Ⅱ, astragaloside Ⅳ, calycosin-7-glucoside, formononetin and 7,2'-dihydroxy-3',4'-dimethoxyisoflavan were 0.306-0.922 mg·g-1, 0.053-0.183 mg·g-1, 0.015-0.092 mg·g-1, 0.069-0.823 mg·g-1, 0-0.098 mg·g-1 and 0.020-0.107 mg·g-1 in 20 batches of A. membranaceus, respectively. Using astragaloside Ⅱ as an internal reference, the relative correlation factors of astragaloside Ⅰ, astragaloside Ⅳ, calycosin-7-O-β-D-glucoside, formononetin, and 7,2'-dihydroxy-3',4'-dimethoxyisoflavan were calculated as 0.561, 0.835, 0.299, 0.796, and 0.799, respectively. The results were compared with those obtained by the external standard method to verify the feasibility, rationality and repeatability of QAMS method, and there was no significant difference in assay results between the two methods. In conclusion, the QAMS method is accurate and feasible, and could be used to determine the contents such as astragaloside Ⅰ, astragaloside Ⅱ, astragaloside Ⅳ, calycosin-7-glucoside, formononetin and 7,2'-dihydroxy-3',4'-dimethoxyisoflavan, and it can be used for quality control of A. membranaceus.
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A quantitative analytical method for multi-components with a single-marker (QAMS) was established for simultaneous determination of neochlorogenic acid, chlorogenic acid, caffeic acid, cryptochlorogenic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in Artemisia capillaris Thunb standard decoction. The separation was performed on a Waters CORTECS T3 column (2.1 mm × 100 mm, 2.7 μm), with the mobile phase consisting of 0.05% phosphate acid solution-acetonitrile for gradient elution. The column temperature was 30 ℃, and flow rate was 0.5 mL·min-1. Using chlorogenic acid as an internal reference, the relative correlation factors of neochlorogenic acid, caffeic acid, cryptochlorogenic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid were calculated following UHPLC, as 0.928 0, 0.546 2, 1.099 8, 0.872 1, 1.086 8, 0.739 2, 1.056 6, respectively. The results were compared with those obtained by the external standard method to verify the feasibility, rationality and repeatability of QAMS method. There was no significant difference in assay results between QAMS and the external standard method. In conclusion, the QAMS method is accurate and feasible, and could be used to determine the content such as neochlorogenic acid, caffeic acid, cryptochlorogenic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in Artemisia capillaris Thunb standard decoction.
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Objective: To establish a new method for the quantitative analysis of multi-components by single-marker (QAMS) to simultaneous determine six naphthoquinone components in Arnebia euchroma. Methods: The chromatographic peaks of the main naphthoquinone components in A. euchroma were identified by high resolution LC-MS. The acetyl Shikonin was used as internal marker to calculate the relative correlation factors (RCF) of deoxyshikonin, isobutyrylshikonin, β-acetoxyisovalerate shikonin, and β,β'-dimethylacryloyl shikonin by HPLC, and examine the durability and reproducibility of the RCF. The external standard method and QAMS were compared to determine the six components in A. euchroma. Results: The repeatability of RCF was good. The results calculated with QAMS were consistent with the results by the external standard method. Conclusion: The QAMS method for simultaneously measuring the content of six components is accurate and reliable to evaluate the quality of A. euchroma.
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Objective: To establish a method for the quantitative analysis of multi-components by single marker (QAMS)to determine the content of rutin,linarin,luteolin and apigenin in Herba Cirsii. Methods: Rutin was selected as the internal reference substance,the relative correction factors(RCF)of the other 3 components were established re- spectively,and then the content of each component was calculated according to respective RCF. At the same time,the external standard method was used to determine the content of all four components in Herba Cirsii,the differences be- tween calculated values and measured values were compared,and the method validation was performed. Results: No significant difference was observed between the calculated values and measured ones according to the results from ten batches of Herba Cirsii samples. Conclusion: The validated QAMS method is proved to be of good precision,reproduc- ibility,and reliability which is suitable and feasible for the quality control of Herba Cirsii
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Objective To establish a quantitative analysis of multi-component with a single-marker (QAMS) method for the quality control of Wuzi Yanzong Pills (WYP). Methods Six main effective components (schisandrin, hyperin, quercitrin, kaempferol 3-O-rutinoside, deoxyschizandrin, and γ-schizandrin) of WYP were simultaneously separated on a reversed-phase column (Ultimate LP-C18) with high-resolution of each chromatographic peak by high performance liquid chromatography (HPLC). Schisandrin was selected as the internal reference, and the relative correlation factors (RCFs) of other five components were calculated to achieve QAMS. The ruggedness of RCFs was tested on different instruments and columns. Moreover, results of the QAMS were compared with the external standard method. Results Within a certain linear range, the RCFs of hyperin, quercitrin, kaempferol 3-rutinoside, deoxyschizandrin, and γ-schizandrin were 0.36, 4.86, 0.88, 7.34, and 6.35, respectively. The repeatability was good under different experimental conditions. There were no significant differences between the calculated value and estimated value on QAMS and external standard method. Conclusion The QAMS method can be used to assay the content of six components of WYP simultaneously and control the quality of WYP simplely, reliably, and accurately.
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Objective To establish a method for the quantitative analysis of multi-component with a single-marker (QAMS) to determine the contents of four rhubarb anthraquinones inShanzha Xiaozhi capsules; To conduct methodology investigation.Methods Emodin was set as the internal reference substance, and the relative correlation factors of aloe emodin, rhein, chrysophanol to emodin were calculated and evaluated. The contents of these four anthraquinones were determined by the external standard method and QAMS, respectively. Rationality, feasibility and repeatability of the QAMS method were verified by comparing the results obtained from the two different methods. Results The QAMS method and HPLC method did not show significant difference in results.Conclusion QAMS method can be used as a quality assessment model for quantity evaluation of anthraquinones inShanzha Xiaozhi Capsules.
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OBJECTIVE: To develop a method of quantitative analysis of multicomponents by singlemarker (QAMS) for simultaneous determination of six oligosaccharide esters in Polygalae Radix. METHODS: 3, 6'-Disinapoyl sucrose (DISS) was used as the internal reference substance. The relative correlation factors (RCF) of sibiricose A5, sibiricose A6, tenuifoliside B, tenuifoliside A, and tenuifoliside C to DISS were calculated. The contents of the six oligosaccharide esters in 20 different batches of Polygalae Radix were determined by both external standard method and QAMS. The assay results were compared to evaluate the accuracy of QAMS method. RESULTS: The RCF of sibiricose A5, sibiricose A6, tenuifoiside B, tenuifoliside A, and tenuifoiside C to DISS were 1.23, 1.16, 1.56, 1.79, and 0.92, respectively. Under different experimental conditions, the RCF had good reproducibility, and there was no significant difference in the quantitative analysis results between the external standard method and QAMS method. CONCLUSION: The QAMS method is feasible and credible, and can be used for the quality control of oligosaccharide esters in Polygalae Radix.
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OBJECTIVE: To establish a auantitative analysis of multi-components by single marker(QAMS) method for determining the content of anthraquinones in Dabaidu capsules(traditional Chinese medicines). METHODS: The relative correction factors (RCFs) of emodin to rhein, chrysophanol, and physcion were determined on a C18 column (4.6 mm × 250 mm, 5μm) with methanol-0.1% phosphoric acid (85:15) as mobile phase. The flow rate was 1.0 mL · min-1. The column temperature was set at 25°C. The detection wavelength was set at 254 nm. The relative retention time (RRT) was used to define the target peak. RESULTS: The calibration curves for rhein, emodin, chrysophanol, and physcion showed good linearity in the ranges of 5.724 × 10-2-0.5724 μg, 4.584 × 10-2 -0.4584 μg, 6.368 × 10-2-0.6368 μg and 6.548 × 10-2 - 0.6548 μg, r(0.9997-0.9999), respectively. The recoveries were (97.8 ± 1.4)%, (100.7 ± 1.0)%, (98.6 ± 0.6)% and (97.0 ± 1.4)%, respectively. The RCFs were 1.19, 1.40 and 1.06. CONCLUSION: The method is simple, rapid, accurate and reliable. It can be applied to control the quality of anthraquinones in Dabaidu capsules.
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Objective To explore the role of anterior horn index (AHI) in magnetic resonance imaging (MRI) outcome after intraventricular hemorrhage (IVH) and the correlation factors of IVH in preterm infants.Methods We selected 55 preterm infants with IVH(Ⅱ-Ⅳ degree) confirmed by MRI as case group,and 55 preterm infants with normal MRI as control group,all the cases were admitted in neonatal medical wards in our hospital from January 2009 to September 2011.Then 30 infants of case group were scaned for the second time,to see if they had posthemorrhagic ventricular dilatation (PHVD) and compare the AHI with the last time MRI,and analysis the related factors associated with IVH in preterm infants.Results MRI results:9 infants had PHVD,and the AHI rised (0.36 ± 0.05 v s 0.46 ± 0.06,P ＜ 0.01);21 infants didn't have PHVD,and the AHI decreased (0.31 ± 0.02 vs 0.30 ± 0.02,P ＜ 0.01).Logistic regression analysis:vaginal delivery,use of pulmonary surfactant,mechanical ventrilator care were associated with IVH (P ＜0.05).Conclusion AHI can be used for judging PHVD in preterm infants with IVH.Preterm infants with IVH,especially grade Ⅱ should be monitored AHI.Vaginal delivery and mechanical ventrilator may be risk factor of IVH in preterm infants,while use of pulmonary surfactant may a protective factor of IVH.
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OBJECTIVE: To establish a quality evaluation method, quantitative analysis of multi-components with a single-marker(QAMS), to simultaneously determine the contents of four flavones in Yinhuang preparations. METHODS: Baicalin was used as the internal reference substance. The relative correlation factors(RCF) of wogonoside, baicalein and wogonin to baicalin were calculated and established. The contents of these four flavones were determined by the external standard method and QAMS respectively. The QAMS method was validated through comparison of the results obtained by the two different methods. RESULTS: Within the linear ranges, the values of RCF determined at 274 nm of wogonoside, baicalein and wogonin to baicalin were 1.20, 1.62 and 1.68 respectively. The RCF showed good reproducibility within the range of 1.7%-4.8% for the three studied compounds. The two methods did not show significant difference in assay results. CONCLUSION: The QAMS method is feasible, credible, and can be used to determine multiple flavones in Yinhuang preparations.
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OBJECTIVE: To study the feasibility of quantitative analysis of multi-components by single-marker (QAMS) in determination of flavones in Shuanghuanglian preparations by comparing the results assayed by QAMS and external standard method (ESM). METHODS: The analysis was performed on a Xtimate™ C18 column (4.6 mm × 250 mm, 5 μm)with methanol and water containing 0.2% phosphoric acid as the mobile phase in gradient elution. The flow rate was set at 1.0 mL · min-1. The UV detector wavelength was 274 nm, and the column temperature was 30°C. RESULTS: Within the linear ranges, the analysis results showed no significant difference between QAMS and ESM. CONCLUSION: The QAMS method is credible and will be a new quality evaluation pattern for the determination of multiple flavones in Shuanghuanglian preparations.
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Objective To investigate the correlation factors for hot flashes in climacteric women so as to improve their quality of life. Methods A questionnaire was distributed among 792 climacteric women. The questionnaire items included general information, characteristics of menstruation, reproduction history, information of hot flashes, lifestyle and sexual life. Weight, height, blood pressure, waist and hip circumferences were measured. Logistic regression analysis was made to find out the correlation factors for hot flashes. Results Univariate logistic regression analysis showed that age, education, income, present characteristics of menstruation, dietary habit, soy products intake, physical exercise, satisfaction of sexual life, body mass index (BMI) and systolic blood pressure were significantly associated with hot flashes. Multivariate logistic regression analysis revealed that irregular menstruation or menopause, frequent exercise and higher systolic blood pressure were risk factors for hot flashes in climacteric women. However, higher education, soy products intake and satisfaction of sexual life were protection factors. Conclusion Guiding climacteric women to take moderate physical exercise, knowing more about climacterium, consuming more soy products and controlling diastolic blood pressure are likely to decrease the incidence of hot flashes.
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OBJECTIVE To analyze the correlation factors and preventive measures of lower respiratory tract infection after general anesthesia with laryngeal mask airway.METHODS In surgery 158 cases of general anesthesia with laryngeal mask ventilation in gynecology and orthopedic wards were analyzed retrospectively.RESULTS Forty-three cases were found in hospitals with lower respiratory tract infection,accounting for 83% of the total lower respiratory tract infection in the wards at the same period.CONCLUSIONS The anesthesia way,the age,the operation time,whether gastric tube,the abuse of antibiotics and whether the anesthesia equipments strictly disinfected and managed may be the important factors which can cause the lower respiratory tract infection.
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@#ObjectiveTo investigate the clinical characteristics of post-stroke depression (PSD) and correlative factors.Methods116 patients with PSD were randomly divided into the treatment group (n=69) and control group (n=47). The patients of the treatment group were treated with selective serotonin re-uptake inhibitors (SSRI) 20 mg/d and combined with psychological counselling, those of the control group only with SSRI. All patients were examined with Self-rating Depression Scale (SDS) when admission and one month after treatment.ResultsAfter treatment, the SDS scores of all patients in two groups decreased ( P<0.05～0.01), but the therapeutic effect of the treatment group was better than that of the control group ( P<0.05).ConclusionThe PSD is correlated with psych-social circumstances except body disease.
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OBJECTIVE To investigate the incidence of hospital infection in surgery intensive care unit(SICU) and to provide evidence for controlling hospital infection.METHODS Managers of hospital infection entered SICU from Jan to Jun in 2007 and monitored items of inpatients who stayed more than two days,such as incidence of hospital infection,infective sites,underlying diseases,invasive operation and time in hospital.Patients had been followed up for 2 days after moving out of SICU.RESULTS In 341 cases,the total incidence of hospital infection was 19.64%(67/341).Constituent ratio:respiratory tract infection was 68.65%(46/67),and urinary tract infection was 19.4%(13/67).Incidence of hospital infection in patients older than 60 years was 48.24%(41/85)and in patients younger than 60 years was 10.16%(26/256).There was significant difference between these two groups(?2= 58.60,P
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[Objective]To determine the incidence and factors associated with pyrexia after total knee replacement(TKR).[Method]A retrospective review of 200 patients in departments of orthopaedics of our hospital and West China hospital who underwent TKRs from August 1998 and April 2006 was performed.The clinical,surgical and follow-up data were abstracted from the patients' records and were analyzed with SPSS 12.0.[Result]There was a statistically significant increase of the mean temperature post-operations,which remained significant throughout the first 7 days after surgeries(P
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Objective:To supply the reliable evidence for a further research of the TCM syndrome of hyperuricemia and treating it with traditional Chinese medicine,we try to verification the risk factors and to research TCM syndrome differentiation for diagnosis in patients with hyperuricacidemia.Methods: We selected cases according to the standard of hyperuricacidemia's diagnose and adopted the method of retrospective and prospective analysis.Each of cases was investigated and filled in the form.These data were processed through statistical method in the end.Results: Clinical investigation selected 109 cases patients with hyperuricacidemia.First,male group incidence rate was higher than female.Second,incidence rate was increasing with age(r=0.826 p
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Objective To investigate the circumstance of hospital-acquired infection in patients with acute myocardial infarction(AMI),analyse the correlation factors of hospital-acquired infection and guide the clinical treatment in AMI patients.Methods To retrospectively analyse the relation between the incidence rate of hospital-acquired infection and the factors such as sex,age,heart function,position of mycardiac infarction,complication,virulence operation,the use of antibiotics in 254 patients with AMI.Results The factors of advanced age,cardia insufficiency,anterior wall and multitude walls mycardiac infarction,delay visit,chronicity pulmonary disease,diabetes mellitus,virulence operation could increase the incidence rate of hospital-acquired infection(P
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OBJECTIVE:To study the correlation between MTX concentration and hydration volume,alkalization volume,urine volume,body weight and biochemical indexes in Children with acute lymphoblastic leukemia(ALL).METHODS:The case histories of pediatric ALL patients hospitalized between 2003 and 2006 who were treated with MTX antitumor thrapy were reviewed retrospectively for a recording of MTX concentration,hydration volume,alkalization volume,urine volume,body weight and biochemical indexes etc.The data were analyzed with multiple linear regression method by SPSS(10.0)software.RESULTS:No significant correlation was noted between log MTX concentration and most of the biochemical indexes,hydration volume,alkalization volume and urine volume;however,log MTX concentration at 24 h was negatively correlated with body weight but positively correlated with TBIL(total bilirubin)(P